MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation.
方法采用RP - HPLC法,梯度洗脱,研究决明子饮片的指纹图谱,并作相似度比较分析。
Methods: The gradient elution mode was applied in chromatographic separation and data were analyzed to develop HPLC fingerprint analysis of Safflor Yellow.
方法:采用梯度洗脱的方法进行色谱分离,对各色谱峰进行数据处理,得出红花黄色素的指纹图谱。
The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化,提出并应用了流动相速度梯度洗脱方法。
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